Composite

Part:BBa_K3610038:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-05)


BAK1 ectodomain / LargeBit for S. cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 848
    Illegal PstI site found at 893
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 848
    Illegal PstI site found at 893
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1278
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 848
    Illegal PstI site found at 893
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 848
    Illegal PstI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed localization at the membrane, thus we needed a signal peptide native to C. reinhardtii.

The self-cleaving peptide from the human foot-and-mouth-disease virus allows expression of multiple protein coding fragments with the same promoter.

Source

BBa_K319005, BBa_K2809002, BBa_K3610003, BBa_K1150016, BBa_K416001, BBa_K3610014, BBa_K2753050

References

Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.

England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.

Koller, Teresa; Bent, Andrew F. (2014): FLS2-BAK1 extracellular domain interaction sites required for defense signaling activation. In: PLOS ONE 9 (10), e111185-e111185. DOI: 10.1371/journal.pone.0111185.

Ranf, Stefanie (2018): Pattern Recognition Receptors—Versatile Genetic Tools for Engineering Broad-Spectrum Disease Resistance in Crops. In: Agronomy 8, S. 134. DOI: 10.3390/agronomy8080134.